Macrozoobenthos-Based Biomonitoring of River Water Quality Using BMWP–ASPT: Evidence from Kali Jarak, East Java, Indonesia
DOI:
https://doi.org/10.51264/inajl.v7i1.140Keywords:
Bioindicator, BMWP-ASPT, Kali Jarak, Macrozoobenthos, Water qualityAbstract
Macrozoobenthos are key benthic invertebrates that play an essential role in nutrient cycling and are widely recognized as biological indicators of freshwater ecosystem health. However, freshwater ecosystems are increasingly exposed to anthropogenic pressures, including domestic activities, agricultural practices, and other human disturbances, which may contribute to changes in water quality and aquatic habitat conditions. Such pressures highlight the need for biological assessments that can capture ecological responses to environmental changes. This study assessed the diversity of macrozoobenthos in Kali Jarak, Wonosalam District, East Java, to evaluate its potential as a bioindicator of water quality. Samples were collected using purposive sampling across three stations with three replications. Identification was performed to the genus level, and data were analyzed using Shannon–Wiener diversity index, dominance index, and the Biological Monitoring Working Party–Average Score per Taxon (BMWP–ASPT) method. A total of 19 genera were recorded. Diversity index values ranged from 1.19 to 1.70, while dominance index values ranged from 0.27 to 0.43. The BMWP scores (47–66) and ASPT values (6.0–6.7) indicated that all stations were in the “excellent” category of water quality. Correlation analysis revealed significant relationships between certain genera and physico-chemical parameters: temperature with Austrotinodes and Macrelmis, pH and discharge with Baetis, current with Dicranota, BOD with Prodiamesa and Rhitrogena, and COD with Euphaea. Overall, the findings confirm that macrozoobenthos diversity provides a sensitive and reliable biological indicator for monitoring river water quality, supporting its application in freshwater biomonitoring and sustainable watershed management.
References
Ab Hamid, S., & Rawi, C. S. M. (2017). Application of aquatic insects (Ephemeroptera, Plecoptera and Trichoptera) in water quality assessment of Malaysian headwater. Tropical life sciences research, 28(2), 143. http://doi.org/10.21315/tlsr2017.28.2.11
Afwa, R. S., Muskananfola, M. R., Rahman, A., Suryanti, S., & Sabdaningsih, A. (2021). Analysis of the load and status of organic matter pollution in Beringin River Semarang. Indonesian Journal of Chemical Science, 10(3), 168-178. https://doi.org/10.15294/ijcs.v10i3.50705
Aissani, S., Hamil, S., Bouhenna, M. M., & Baha, M. (2026). Response of aquatic insects to physicochemical parameters in two reservoirs in the tipaza province (Algeria). Euro-Mediterranean Journal for Environmental Integration, 11(5), 195. https://doi.org/10.1007/s41207-026-01159-z
Albini, D., Lester, L., Sanders, P., Hughes, J., & Jackson, M. C. (2023). The combined effects of treated sewage discharge and land use on rivers. Global Change Biology, 29, 6415–6422. https://doi.org/10.1111/gcb.16934
Amaral, P. H. M. D., Silveira, L. S. D., Rosa, B. F. J. V., Oliveira, V. C. D., & Alves, R. D. G. (2015). Influence of habitat and land use on the assemblages of Ephemeroptera, Plecoptera, and Trichoptera in neotropical streams. Journal of Insect Science, 15(1), 60. https://doi.org/10.1093/jisesa/iev042
Anwar, Y. W. (2025). Pengaruh Pencemaran Air Sungai terhadap Kesehatan Lingkungan dan Masyarakat: Literature review. Jurnal Kesehatan Tambusai, 6(2), 5061-5073. https://doi.org/10.31004/jkt.v6i2.43127
Aydemir, H. B., Mercan, D., Arslan, N., & Aydemir, M. N. (2025). Machine Learning?Based Bioindicator Assessment: Driver Forces of Chironomidae Community Structure in the Eastern Black Sea Basin. Ecohydrology, 18(5), e70093. https://doi.org/10.1002/eco.70093
Badan Pusat Statistik Kabupaten Jombang. (2026). Kabupaten Jombang dalam angka 2026 (Volume 46). BPS Kabupaten Jombang.
Baker, K., Chadwick, M. A., Kahar, R., Sulaiman, Z. H., & Wahab, R. A. (2016). Fluvial biotopes influence macroinvertebrate biodiversity in South?East Asian tropical streams. Ecosphere, 7(12). https://doi.org/10.1002/ecs2.1479
Bartram, J., & Ballance, R. (Eds.). (1996). Water quality monitoring: a practical guide to the design and implementation of freshwater quality studies and monitoring programmes. CRC Press.
Benítez-Álvarez, L., Leria, L., Sluys, R., Leal-Zanchet, A. M., & Riutort, M. (2023). Niche modelling and molecular phylogenetics unravel the colonisation biology of three species of the freshwater planarian genus Girardia (Platyhelminthes, Tricladida). Hydrobiologia, 850(14), 3125-3142. https://doi.org/10.1007/s10750-023-05239-x
Boonsoong, B., & Braasch, D. (2013). Heptageniidae (Insecta, Ephemeroptera) of Thailand. ZooKeys, (272), 61. https://doi.org/10.3897/zookeys.272.3638
Braun, B. M., Pires, M. M., Kotzian, C. B., & Spies, M. R. (2014). Diversity and ecological aspects of aquatic insect communities from montane streams in southern Brazil. Acta Limnologica Brasiliensia, 26, 186-198. https://doi.org/10.1590/S2179-975X2014000200009
Braun, B. M., Salvarrey, A. V. B., Kotzian, C. B., Spies, M. R., & Pires, M. M. (2014). Diversity and distribution of riffle beetle assemblages (Coleoptera, Elmidae) in montane rivers of Southern Brazil. Biota Neotropica, 14(2). https://doi.org/10.1590/1676-060320140615183046
Bridgewater, L. L. (2017). Standard methods for the examination of water and wastewater. American public health association.
Cahyani, N. T., Chazanah, N., Wulandari, S., Baranovtaria, B., Fahmi, M. R. F., & Fikri, M. H. (2026). Macrozoobenthos as Bioindicators of Water Quality in a Managed and Restored Tropical River: A Case Study of the Upper Citarum River. Jurnal Teknik Lingkungan, 32(1), 95-106. https://doi.org/10.5614/j.tl.2026.32.1.10
Cañedo-Argüelles, M., Bogan, M. T., Lytle, D. A., & Prat, N. (2016). Are Chironomidae (Diptera) good indicators of water scarcity? Dryland streams as a case study. Ecological Indicators, 71, 155-162. https://doi.org/10.1016/j.ecolind.2016.07.002
Chakraborty, A., Saha, G. K., & Aditya, G. (2022). Macroinvertebrates as engineers for bioturbation in freshwater ecosystem. Environmental Science and Pollution Research, 29(43), 64447-64468. https://doi.org/10.1007/s11356-022-22030-y
Chakravarty, T., & Gupta, S. (2024). Aquatic insects as indicators of water quality: Seasonal distribution and biomonitoring insights from a hilly river in the Eastern Himalayan region, India. Cleaner Water, 2, 100056. https://doi.org/10.1016/j.clwat.2024.100056
Dudgeon, D. (1989). Life cycle, production, microdistribution and diet of the damselfly Euphaea decorata (Odonata: Euphaeidae) in a Hong Kong forest stream. Journal of Zoology, 217(1), 57-72. https://doi.org/10.1111/j.1469-7998.1989.tb02474.x
Eichert, A., De Almeida, L. H., Du, Y. Z., Duarte, T., Fochetti, R., Hotaling, S., ... & Ware, J. (2025). Stonefly systematics: past, present, and future. Insect systematics and diversity, 9(4), 7. https://doi.org/10.1093/isd/ixaf026
Fabanjo, M. A., & Inayah, I. (2021). Analysis of water quality in the Mandaong River for development of freshwater fish cultivation. Jurnal Biologi Tropis, 21(3), 965-973. https://doi.org/10.29303/jbt.v21i3.2785
Feio, M. J., Hughes, R. M., Serra, S. R., Nichols, S. J., Kefford, B. J., Lintermans, M., ... & Sharma, S. (2022). Fish and macroinvertebrate assemblages reveal extensive degradation of the world's rivers. Global Change Biology, 29(2), 355-374. https://doi.org/10.1111/gcb.16439
Ficsór, M., & Csabai, Z. (2021). Longitudinal zonation of larval Hydropsyche (Trichoptera: Hydropsychidae): abiotic environmental factors and biotic interactions behind the downstream sequence of Central European species. Hydrobiologia, 848(15), 3371-3388. https://doi.org/10.1007/s10750-021-04602-0
Garner, G., Malcolm, I. A., Sadler, J. P., & Hannah, D. M. (2017). The role of riparian vegetation density, channel orientation and water velocity in determining river temperature dynamics. Journal of Hydrology, 553, 471-485. https://doi.org/10.1016/j.jhydrol.2017.03.024
Gerber, A., & Gabriel, M. J. M. (2002). Aquatic Invertebrates of South African Rivers. Institute of Water Quality Studies, Pretoria.
González-Córdoba, M., del-Carmen-úñiga, M., Giraldo, L. P., Ramírez, Y. P., & Chará, J. (2020). Sensitivity of Elmidae (Insecta: Coleoptera) to habitat disturbance and physicochemical water quality in lotic environments of the Colombian Andes. Revista De Biología Tropical, 68(2), 601-622. https://doi.org/10.15517/rbt.v68i2.36702
Harrison, S. S. C., Pretty, J. L., Shepherd, D., Hildrew, A. G., Smith, C., & Hey, R. D. (2004). The effect of instream rehabilitation structures on macroinvertebrates in lowland rivers. Journal of applied Ecology, 41(6), 1140-1154. https://doi.org/10.1111/j.0021-8901.2004.00958.x
Helard, D., Indah, S., & Wilandari, M. (2021). Spatial variation of electrical conductivity, total suspended solids, and total dissolved solids in the Batang Arau River, West Sumatera, Indonesia. IOP Conference Series: Materials Science and Engineering (Vol. 1041, No. 1, p. 012027). IOP Publishing. https://doi.org/10.1088/1757-899X/1041/1/012027
Kadim, M. K., & Pasisingi, N. (2024). Kondisi habitat fisik dan keanekaragaman makroinvertebrata sebagai indikator pencemaran di sungai Bone Gorontalo. Jurnal Kesehatan Lingkungan Indonesia, 23(3), 301-310. https://doi.org/10.14710/jkli.23.3.301-310
Krebs, C. J. (1985). Ecology: The Experimental Analysis of Distribution and Abundance. Harper & Row Publishers. New York.
Lencioni, V., Marziali, L., & Rossaro, B. (2012). Chironomids as bioindicators of environmental quality in mountain springs. Freshwater Science, 31(2), 525-541. https://doi.org/10.1899/11-038.1
Li, X., Yan, L., Zhi, X., Hu, P., Shang, C., & Zhao, B. (2025). Characteristics of the macroinvertebrate community structure and their habitat suitability conditions in the Chishui River. Frontiers in Ecology and Evolution, 12, 1459468. https://doi.org/10.3389/fevo.2024.1459468
Madsen, P. B., Morabowen, A., Andino, P., Espinosa, R., CAUVY?FRAUNIÉ, S. O. P. H. I. E., Dangles, O., & Jacobsen, D. (2015). Altitudinal distribution limits of aquatic macroinvertebrates: an experimental test in a tropical alpine stream. Ecological Entomology, 40(5), 629-638. https://doi.org/10.1111/een.12232
Mandaville, S. M. (2002). Benthic macroinvertebrates in freshwaters: Taxa tolerance values, metrics, and protocols (Vol. 128). Nova Scotia: Soil & Water Conservation Society of Metro Halifax.
Marpaung, S. M., Muhammad, F., & Hidayat, J. W. (2014). Keanekaragaman dan kemelimpahan larva insekta akuatik sebagai bioindikator kualitas air di Sungai Garang, Semarang. Jurnal Biologi, 3(4), 1-8.
Mebane, C. A., Schmidt, T. S., Miller, J. L., & Balistrieri, L. S. (2020). Bioaccumulation and toxicity of cadmium, copper, nickel, and zinc and their mixtures to aquatic insect communities. Environmental Toxicology and Chemistry, 39(4), 812-833. https://doi.org/10.1002/etc.4663
Muntalif, B. S., Chazanah, N., Ilmi, F., Sari, N. E., & Bagaskara, S. W. (2023). Distribution of the riverine benthic macroinvertebrate community along the citarum cascading dam system in West Java, Indonesia. Global Ecology and Conservation, 46, e02580. https://doi.org/10.1016/j.gecco.2023.e02580
Nadhifah, F. (2025). Hubungan Indeks Keanekaragaman Makrozoobentos dengan Kualitas Air di Hulu Sungai Brantas, Wonosalam Kabupaten Jombang. Environmental Pollution Journal, 5(1), 63-74. https://doi.org/10.58954/epj.v5i1.241
Odum, E. P. (1993). Dasar-dasar Ekologi. Terjemahan T. Samingan. Gadjah Mada University Press. Yogyakarta.
Oscoz, J., Galicia, D., & Miranda, R. (2011). Identification Guide of Freshwater Macroinvertebrates of Spain. Springer, New York.
Patang, F., Soegianto, A., & Hariyanto, S. (2018). Benthic macroinvertebrates diversity as bioindicator of water quality of some rivers in East Kalimantan, Indonesia. International Journal of Ecology, 2018(1), 5129421. https://doi.org/10.1155/2018/5129421
Pires, M. M., Müller, N. F. D., Stenert, C., & Maltchik, L. (2020). Influence of different riparian vegetation widths and substrate types on the communities of larval Odonata (Insecta) in southern Brazilian streams. Acta Limnologica Brasiliensia, 32, e301. https://doi.org/10.1590/S2179-975X2520
Prakoso, S. B., Miyake, Y., Ueda, W., & Suryatmojo, H. (2023). Impact of land use on water quality and invertebrate assemblages in Indonesian streams. Limnologica, 101, 126082. https://doi.org/10.1016/j.limno.2023.126082
Rak, A. E., & Samsudin, M. F. (2025). Determination of the macroinvertebrates benthic ecological index for river health assessment. Journal of Tropical Resources and Sustainable Science (JTRSS), 13(2), 404-411. https://doi.org/10.47253/jtrss.v13i2.1958
Rufusova, A., Beracko, P., Bulánková, E., Derka, T., Kalaninová, D., Korte, T., & Stloukalová, V. (2017). Benthic Invertebrates and Their Habitats. Comenius University in Bratislava, Bratislava.
Schmitt, R., Siegloch, A. E., da Silva, A. L. L., Lisboa, L. K., & Petrucio, M. M. (2016). Temporal variation in the Ephemeroptera, Plecoptera and Trichoptera community in response to environmental drivers in a subtropical stream. Journal of Insect Biodiversity, 4(19), 1-12. https://doi.org/10.12976/jib/2016.4.19
Sotomayor, G., Romero, J., Ballari, D., Vázquez, R. F., Ramírez-Morales, I., Hampel, H., ... & Goethals, P. L. (2023). Occurrence prediction of riffle beetles (Coleoptera: Elmidae) in a tropical Andean basin of Ecuador using species distribution models. Biology, 12(3), 473. https://doi.org/10.3390/biology12030473
Stankovi?, V. M., Jäch, M. A., Vu?kovi?, I., Popija?, A., Kerovec, M., & Ku?ini?, M. (2018). Ecological traits of water beetles in a karstic river from the Eastern Mediterranean region. Limnologica, 71, 75-88. https://doi.org/10.1016/j.limno.2018.06.002
Sudarso, J., Ibrahim, A., Sugiarti, S., Riani, E., Mayaningtyas, P., Yamin, M., ... & Utami, R. R. (2025). Rapid assessment implementation in the development of biocriteria and organic enrichment evaluation in the Citarum River, Indonesia. International Journal of Environmental Studies, 82(2), 871-890. https://doi.org/10.1080/00207233.2024.2409044
Sulistiyowati, E., & Awaliyah, D. F. (2025). Assessing Health Status of Gajahwong River Ecosystem in Yogyakarta City through Biomonitoring and Community Empowerment. Jurnal Ilmu Lingkungan, 23(4), 1046-1055. https://doi.org/10.14710/jil.23.4.1046-1055
Sumudumali, R. G. I., & Jayawardana, J. M. C. K. (2021). A review of biological monitoring of aquatic ecosystems approaches: with special reference to macroinvertebrates and pesticide pollution. Environmental management, 67(2), 263-276. https://doi.org/10.1007/s00267-020-01423-0
Tampo, L., Kaboré, I., Alhassan, E. H., Ouéda, A., Bawa, L. M., & Djaneye-Boundjou, G. (2021). Benthic macroinvertebrates as ecological indicators: their sensitivity to the water quality and human disturbances in a tropical river. Frontiers in Water, 3, 662765. https://doi.org/10.3389/frwa.2021.662765
Tanaka, Y., Minggat, E., & Roseli, W. (2021). The impact of tropical land-use change on downstream riverine and estuarine water properties and biogeochemical cycles: a review. Ecological processes, 10(1), 40. https://doi.org/10.1186/s13717-021-00315-3
Wibowo, D. N., Setijanto, S., & Santoso, S. (2017). Benthic macroinvertebrate diversity as biomonitoring of organic pollutions of river ecosystems in Central Java, Indonesia. Biodiversitas, 18(2), 671-676. https://doi.org/10.13057/BIODIV/D180233
Zeybek, M., Kalyoncu, H., Karaka?, B., & Özgül, S. (2014). The use of BMWP and ASPT indices for evaluation of water quality according to macroinvertebrates in De?irmendere Stream (Isparta, Turkey). Turkish Journal of Zoology, 38(5), 603-613. https://doi.org/10.3906/zoo-1310-9
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Hernanda Afra Haniyyah, Dwi Suheriyanto, Eko Budi Minarno, Indana Zulfa Fauzia

This work is licensed under a Creative Commons Attribution 4.0 International License.




